Uyu mutsara unobvunza mubvunzo uyu: "Zvakanaka, rudzii rwe cryogenic vial iyi kana isingakwanise kushandiswa mumvura ine nitrogen?"
Hapana vhiki rinopfuura isu tisingabvunzwe kuti titsanangure ichi chinoratidzika sechisinganzwisisike chirevo chinoonekwa pane yega yega chigadzirwa peji zvisinei nemugadziri, zvisinei nehukuru uye zvisinei kana iri yemukati tambo cryovial kana yekunze tambo cryovial.
Mhinduro ndeyokuti: Iyi inyaya yemhosva uye kwete mubvunzo pamusoro pemhando ye cryovial.
Ngatitsanangure.
Kufanana nemachubhu akawanda akasimba erabhoritari, cryovials inogadzirwa kubva kune tembiricha yakagadzikana polypropylene.
Ukobvu hwepolypropylene hunotaridza tembiricha yakachengeteka.
Mazhinji 15mL uye 50mL conical chubhu ane madziro matete anoganhurira kushanda kwawo kumatembiricha asiri pasi pe -86 kusvika -90 Celsius.
Masvingo matete anotsanangurawo kuti sei 15mL uye 50mL conical chubhu isingakurudzirwe kuruka pamitengo nekukurumidza kupfuura 15,000xg sezvo purasitiki iri nyore kutsemuka nekutsemuka kana ikashandiswa kupfuura chikumbaridzo ichi.
Mabhodhoro eCryogenic anogadzirwa kubva kugobvu repolypropylene iro rinovatendera kuti vabate pasi petembiricha inotonhora zvakanyanya uye kurukaniswa mucentrifuge nekumhanya kunopfuura 25,000xg kana kupfuura.
Dambudziko riri nevhavha yekuvharisa inoshandiswa kuchengetedza cryovial.
Kuti cryovial ichengetedze nemazvo matishu, sero kana hutachiona sampuli yairi, iyo kapisi inofanira kupeta pasi zvachose uye kugadzira chisimbiso chisingabvi.
Mukaha mudiki unobvumira kubuda uye kusvibiswa kwengozi.
Painstaking kuedza kunoitwa nevagadziri ve cryovial kugadzira chisimbiso chemhando yepamusoro chinogona kusanganisira silicon o-ring uye/kana gobvu shinda yekupusha chivharo pasi zvizere.
Iyi ndiyo chiyero cheiyo mugadziri we cryovial anogona kuunza.
Pakupedzisira budiriro kana kutadza kweiyo cryovial kuchengetedza sampuli inowira pane lab technician kuona kuti chisimbiso chakanaka chaitwa.
Kana chisimbiso chisina kunaka, uye kunyange mumamiriro ezvinhu apo chivharo chakanyatsovharwa, mvura nitrogen inogona kupinda mu cryovial kana yakanyura mumvura yemvura nitrogen.
Kana sampuli ikanyungudutswa nekukasira, iyo nitrogen yemvura inokura nekukurumidza uye ichaita kuti zviri mukati zvakamanikidzwa kuputika uye kutumira mashadhi epurasitiki mumaoko uye kumeso kwechero ani ane rombo rakanaka kuti ave pedyo.
Naizvozvo, nezvisingawanzo siyana, vagadziri ve cryovial vanoda kuti vagovera vavo varatidze neushingi chirevo chekuti vasashandise cryovials yavo kunze kwechikamu chegasi chenitrogen yemvura (yakatenderedza -180 kusvika -186C).
Unogona kukurumidza kupenya zviri mukati mu cryovial nekuinyudza zvishoma muchikamu che nitrogen;dzakasimba zvakakwana uye hadzizotsemuki.
Unoda kudzidza zvakawanda nezve njodzi dzekuchengeta cryogenic vials mune liquid phase liquid nitrogen?
Hechino chinyorwa kubva kuUCLA's Center for Laboratory Safety inonyora kukuvara nekuda kwekuputika kwe cryovial.
Nguva yekutumira: Kubvumbi-21-2022